Cellular progesterone receptor phosphorylation in response to ligands activating protein kinases
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
Progesterone receptors were immunoprecipitated with monoclonal antibodies KD68 from lysates of human breast carcinoma T47D cells labelled to steady state specific activity with /sup 32/Pi. The 120 kDa /sup 32/P-labelled progesterone receptor band was resolved by polyacrylamide gel electrophoresis and identified by autoradiography. Phosphoamino acid analysis revealed serine phosphorylation, but no threonine or tyrosine phosphorylation. Treatment of the /sup 32/Pi-labelled cells with EGF, TPA or dibutyryl cAMP had no significant quantitative effect on progesterone receptor phosphorylation, though the EGF receptor and the cAMP-dependent protein kinases have been reported to catalyze phosphorylation of purified avian progesterone receptor preparations in cell free systems. Progesterone receptor phosphorylation on serine residues was increased by 2-fold in cells treated with 10 nM progesterone; EGF had no effect on progesterone-mediated progesterone receptor phosphorylation.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 6245973
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 146:3; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Detection of phosphorylated forms of moloney murine leukemia virus major capsid protein p30 by immunoprecipitation and two-dimensional gel electrophoresis
In vivo phosphorylation of progesterone receptors in the T47D sub co human breast cancer cell line
Immunologic analysis of human breast cancer progesterone receptors. 2. Structure, phosphorylation, and processing
Journal Article
·
Thu Dec 31 23:00:00 EST 1987
· J. Virol.; (United States)
·
OSTI ID:5299272
In vivo phosphorylation of progesterone receptors in the T47D sub co human breast cancer cell line
Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6708604
Immunologic analysis of human breast cancer progesterone receptors. 2. Structure, phosphorylation, and processing
Journal Article
·
Tue Sep 22 00:00:00 EDT 1987
· Biochemistry; (United States)
·
OSTI ID:5599134
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GROWTH FACTORS
HORMONES
IMMUNOASSAY
ISOTOPES
KETONES
KINETICS
LIGANDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PREGNANES
PROGESTERONE
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RESPONSE MODIFYING FACTORS
STEROID HORMONES
STEROIDS
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GROWTH FACTORS
HORMONES
IMMUNOASSAY
ISOTOPES
KETONES
KINETICS
LIGANDS
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PREGNANES
PROGESTERONE
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RESPONSE MODIFYING FACTORS
STEROID HORMONES
STEROIDS
TRANSFERASES