Loss of striatal Mu/sub 1/ opiate binding by substantia nigra lesions in the rat

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90480-8· OSTI ID:6244668

Bodnar, R J; Clark, J A; Cooper, M L; Pasternak, G W

Opiate receptors have been identified within the striatum and some have been localized presynaptically to nigrostriatal neurons. Using unilateral ablative lesion of the substantia nigra, the authors examined binding in the ipsilateral and contralateral striata. Lesions significantly lowered both /sup 3/H(D-Ala/sup 2/, MePhe/sup 4/, Gly(ol)/sup 5/) enkephalin (DAGO) and /sup 3/H(D-Ala/sup 2/,Leu/sup 5/) enkephalin (DADL) binding. The inclusion of competitors in these assays revealed a decrease in both mu/sub 1/ and mu/sub 2/ receptors. Mu/sub 1/ binding was slightly more sensitive to the lesioning than mu/sub 2/ binding. Selective mu/sub 1/ and mu/sub 2/ binding assays supported these observations. No change in delta binding was observed in the lesioned striata. These studies raise the possibility that both mu/sub 1/ and mu/sub 2/, but not delta, receptors are localized presynaptically on nigrostriatal neurons.

• Research Organization: Cornell Univ. Medical College, New York, NY (USA)
• OSTI ID: 6244668
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 43:21; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL LOCALIZATION
DRUGS
ENDORPHINS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVE CELLS
NEUROREGULATORS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES