Ethanol from whey: continuous fermentation with a catabolite repression-resistant Saccharomyces cerevisiae mutant

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6243030

Terrell, S L; Bernard, A; Bailey, R B

An alternative method for the conversion of cheese whey lactose into ethanol has been demonstrated. With the help of continuous-culture technology, a catabolite repression-resistant mutant of Saccharomyces cerevisiae completely fermented equimolar mixtures of glucose and galactose into ethanol. The first step in this process was a computer-controlled fed-batch operation based on the carbon dioxide evolution rate of the culture. In the absence of inhibitory ethanol concentrations, this step allowed us to obtain high biomass concentrations before continuous fermentation. The continuous anaerobic process successfully incorporated a cell-recycle system to optimize the fermentor productivity. Under conditions permitting a low residual sugar concentration (less than or equal to 1%), maximum productivity (13.6 g liter /sup -1/ h/sup -1/) was gained from 15% substrate in the continuous feed at a dilution rate of 0.2 h/sup -1/. Complete fermentation of highly concentrated feed solutions (20%) was also demonstrated, but only with greatly diminshed fermentor productivity (5.5 g liter/sup -1/ h/sup -1/). 15 references, 3 figures, 1 table.

• Research Organization: Solar Energy Research Inst., Golden, CO
• OSTI ID: 6243030
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 48:3; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
ALCOHOLS
ANAEROBIC CONDITIONS
BIOCONVERSION
BIOSYNTHESIS
CONTINUOUS CULTURE
DATA
ETHANOL
EXPERIMENTAL DATA
FERMENTATION
FUNGI
HYDROXY COMPOUNDS
INDUSTRIAL WASTES
INFORMATION
MICROORGANISMS
MUTANTS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
PRODUCTIVITY
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SYNTHESIS
WASTES
WHEY
YEASTS