Progesterone--specific binding sites in the kidney of the female baboon

Journal Article · Mon Oct 01 04:00:00 EDT 1984 · J. Urol.; (United States)

OSTI ID:6240565

Weaker, F J; Herbert, D C; Sheridan, P J

The uptake and retention of a radiolabeled synthetic progestin, ORG 2058, was studied in the urinary tract of the female baboon. Four estrogen-primed baboons were injected intravenously with 2.5 micrograms./kg. body weight of 3H-ORG 2058. One animal, which served as a control, received an additional injection of 2.5 mg./kg. body weight of unlabeled progesterone. One hour after the injections, the animals were killed and the kidneys, ureters and urinary bladder were removed and processed for autoradiography. Localization of progestin was observed in the nuclei of the convoluted and straight segments of the distal tubule, the ascending thick limb of the loop of Henle and both cortical and medullary collecting tubules. Connective tissue cells were also labeled in the medulla and cortex of the kidney. An absence of silver grains was noted in the renal corpuscle, all segments of the proximal tubule and the thin loop of Henle. Concentration of the tritiated steroid was not observed in either the ureter or bladder or in any portions of the urinary tract of the control animal. This study suggests that progesterone has a direct effect via a progesterone specific receptor on the various target cells that sequestered the 3H-ORG 2058.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 6240565
• Journal Information: J. Urol.; (United States), Journal Name: J. Urol.; (United States) Vol. 132:4; ISSN JOURA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
APES
AUTORADIOGRAPHY
BODY
CHEMICAL BONDS
HORMONES
KETONES
KIDNEYS
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PRIMATES
PROGESTERONE
RECEPTORS
STEROID HORMONES
STEROIDS
TRITIUM COMPOUNDS
VERTEBRATES