Mesangial cells express PDGF (platelet-derived growth factor) mRNAs and proliferate in response to PDGF

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · American Journal of Physiology; (USA)

OSTI ID:6230095

Shultz, P J; DiCorleto, P E; Silver, B J; Abboud, H E ^[1]

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1. Case Western Reserve Univ., Cleveland, OH (USA) Research Institute of the Cleveland Clinic Foundation, OH (USA)

Platelet-derived growth factor (PDGF) is a potent mitogen for cells of mesenchymal origin and is released and/or synthesized by platelets, macrophages, endothelial cells and rat mesangial cells. In the present investigation, the authors found that human glomerular mesangial cells in culture release a PDGF-like protein which competes for {sup 125}I-PDGF binding to human foreskin fibroblasts and is mitogenic for these fibroblasts. The competing and to a lesser extent the mitogenic activities present in the conditioned medium are partially recognized by an anti-PDGF antibody. Northern blot analysis of poly(A){sup +} RNA from human mesangial cells demonstrates the expression of both PDGF A- and B-chain mRNAs. PDGF also binds to mesangial cells in a specific manner and stimulates DNA synthesis and cell proliferation. These data suggest that a PDGF-like protein secreted by mesangial cells or released from platelets, monocytes, or endothelial cells during glomerular inflammation may function as an autocrine or a paracrine growth factor for these cells. The biological role of PDGF in mediating proliferative and other inflammatory events in the glomerulus remains to be identified.

• OSTI ID: 6230095
• Journal Information: American Journal of Physiology; (USA), Vol. 255:4; ISSN 0002-9513
• Country of Publication: United States
• Language: English

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GROWTH FACTORS
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MESSENGER-RNA
DNA HYBRIDIZATION
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CELL CULTURES
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HETEROCYCLIC COMPOUNDS
HYBRIDIZATION
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INTERMEDIATE MASS NUCLEI
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ISOTOPE APPLICATIONS
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