Sensitivity of Macrocystis Gametophytes to Copper

Technical Report · Fri Aug 04 04:00:00 EDT 1978

DOI:https://doi.org/10.2172/6229963· OSTI ID:6229963

Smith, Barbara M. ^[1]; Harrison, Florence L. ^[1]

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1. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Gametophytes of giant kelp, Macrocystis pyrifera, were exposed to copper in a dish-culture bioassay system. Through their development into gametophytes, newly released spores were exposed continuously to various concentrations of copper to determine its effect on vegetative growth. In addition, gametophytes of different ages were exposed continuously to copper to determine the sensitivity of several developmental stages to copper and the effect of copper on the production of gametes. A one-week pulsed-exposure experiment was performed to determine the recovery period for vegetative growth. In experiments using copper spikes of less than 50 ppb, water samples were analyzed by differential pulse anodic stripping voltammetry to determine the content of labile copper. Complete inhibition of growth occurred when gametophytes were exposed continuously to copper concentrations of 500 ppb or more. Vegetative growth was inhibited significantly by continuous exposure to 50 ppb copper; the production of eggs by female gametophytes was inhibited by spikes of 30 ppb copper; or which 1 ppb was ASV-labile. The growth of 1-, 2-, and 5-wk-old gametophytes was inhibited significantly by 1-wk exposure to 100 ppb copper. The spiked cultures required 2 to 3 wk to recover from pulsed exposures to concentrations between 50 and 200 ppb copper.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE; USNRC
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6229963
• Report Number(s): NUREG/CR--0684; UCRL--52481
• Country of Publication: United States
• Language: English

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