Phosphoenolpyruvate carboxylase from spinach leaf tissue: inhibition by sulfite ion

Journal Article · Tue Jan 01 00:00:00 EST 1974 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.53.6.829· OSTI ID:6229262

Mukerji, S K; Yang, S F

Phosphoenolpyruvate carboxylase, partially purified from spinach (Spinacia oleracea) leaves, is inhibited by SO3S ion. The inhibition is competitive or mixed type with respect to HCO3 (Ki = 17 mM), and noncompetitive with respect to phosphoenolpyruvic acid (Ki = 11 mM), MgS (Ki = 10 mM), and MnS (Ki = 2.4 mM). The inhibitory effect of SO3S is more significant in the presence of MnS than in the presence of MgS . L-Malate, an inhibitor of phosphoenolpyruvate carboxylase activity, and SO3S may bind at the same site on the enzyme. Glyoxal bisulfite and glyoxylate bisulfite are equally effective inhibitors of the enzyme activity as SO3S , but -hydroxypyridinemethanesulfonate is a weak inhibitor. The data are discussed in relation to the physiological effect of the air pollutant (SO2) on plant leaf metabolism. 35 references, 5 figures, 3 tables.

• Research Organization: Univ. of California, Davis, CA (United States)
• OSTI ID: 6229262
• Journal Information: Plant Physiol.; (United States), Vol. 53:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARBOXYLASE
ENZYME ACTIVITY
SULFITES
BIOLOGICAL EFFECTS
SULFUR DIOXIDE
AIR POLLUTION
CATIONS
EXPERIMENTAL DATA
MAGNESIUM COMPOUNDS
MALIC ACID
MANGANESE COMPOUNDS
PYRUVIC ACID
SPINACH
ALKALINE EARTH METAL COMPOUNDS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHARGED PARTICLES
DATA
ENZYMES
FOOD
HYDROXY ACIDS
INFORMATION
IONS
KETO ACIDS
LYASES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PLANTS
POLLUTION
SULFUR COMPOUNDS
SULFUR OXIDES
TRANSITION ELEMENT COMPOUNDS
VEGETABLES
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)