Localization of gamma-glutamyl transpeptidase in hamster buccal pouch epithelium treated with 7,12-dimethylbenz(a)anthracene
The utility of gamma-glutamyl transpeptidase (GGT) was explored as a histochemical marker for chemical carcinogenesis in hamster buccal pouch mucosa. One or both buccal pouches of 18 noninbred male Syrian golden hamsters were treated topically with 0.5% 7,12-dimethylbenz(a)anthracene (DMBA) in mineral oil over 16 weeks to produce numerous epithelial lesions at various stages of neoplastic development. Both buccal pouches of 4 control animals were similarly treated with mineral oil alone. GGT activity was not detectable in untreated pouches or pouches treated with mineral oil alone. With this technique, multiple discrete GGT-stained areas were visible in wholemounts prepared at 1 and 6 weeks after the final application of DMBA. The experimental results were consistent with the hypothesis that the early GGT-stained cell populations are preneoplastic in nature.
- Research Organization:
- Harvard School of Dental Medicine, Boston, MA
- OSTI ID:
- 6228882
- Journal Information:
- J. Natl. Cancer Inst.; (United States), Vol. 67:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOGENESIS
BIOLOGICAL INDICATORS
DIMETHYLBENZANTHRACENE
BIOLOGICAL EFFECTS
NEOPLASMS
DIAGNOSIS
PEPTIDE HYDROLASES
BIOLOGICAL LOCALIZATION
EPITHELIUM
HAMSTERS
MUCOUS MEMBRANES
ANIMAL TISSUES
ANIMALS
AROMATICS
BODY
CONDENSED AROMATICS
DISEASES
ENZYMES
HYDROLASES
MAMMALS
MEMBRANES
ORGANIC COMPOUNDS
PATHOGENESIS
RODENTS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)