Effects of thrombin on the integrity of monolayers of cultured human endothelial cells
Journal Article
·
· Thromb. Res.; (United States)
/sup 51/Cr-prelabelled endothelial cells (EC) in confluent monolayers were incubated in RPMI 1640 + foetal calf serum 20% (v/v) to which purified thrombin was added. Thrombin (greater than or equal to 0.1 NIH U/ml) significantly accelerated /sup 51/Cr-release and caused extensive but reversible cell contraction. Thrombin-exposed EC reacted to a new dose of thrombin with no appreciable shape change, but /sup 51/Cr-efflux was again accelerated. EC exposed to thrombin pretreated with N-bromosuccinimide (modifying the macromolecular site) or phenylmethylsulfonyl fluoride (blocking the serine site) retained normal morphology and did not leak excess amounts of /sup 51/Cr. Antithrombin III also inhibited the effect of thrombin. Pretreatment of EC with either indomethacin, aspirin, sulfinpyrazone, pronase or neuraminidase did not influence the effect of subsequent thrombin exposure.
- Research Organization:
- Research Institute for Internal Medicine, University of Oslo, Norway
- OSTI ID:
- 6225659
- Journal Information:
- Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 27:5; ISSN THBRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550801 -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CELL CULTURES
CHROMIUM 51
CHROMIUM ISOTOPES
COAGULANTS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MORPHOLOGY
NORTH AMERICA
NUCLEI
PEPTIDE HYDROLASES
RADIOISOTOPES
SERINE PROTEINASES
THROMBIN
TISSUES
TRACER TECHNIQUES
USA
550801 -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CELL CULTURES
CHROMIUM 51
CHROMIUM ISOTOPES
COAGULANTS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
EVEN-ODD NUCLEI
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MORPHOLOGY
NORTH AMERICA
NUCLEI
PEPTIDE HYDROLASES
RADIOISOTOPES
SERINE PROTEINASES
THROMBIN
TISSUES
TRACER TECHNIQUES
USA