Reticuloendothelial hyperphagocytosis occurs in streptozotocin-diabetic rats. Studies with colloidal carbon, albumin microaggregates, and soluble fibrin monomers
In contrast to previous studies of diabetic humans and animals, which reported unchanged or depressed function, reticuloendothelial system (RES) hyperphagocytosis of colloidal carbon, /sup 125/I-albumin microaggregates, and /sup 125/I-fibrin monomers were observed in rats as early as 14 days after the induction of diabetes with streptozotocin (STZ). The fact that enhanced phagocytosis by RE macrophages was prevented by chronic insulin replacement therapy indicates that the diabetic internal environment of hyperglycemia and hypoinsulinemia was perhaps responsible for the observed changes. Experiments involving organ localization of intravenously administered particles, perfusion of isolated livers, and microscopic examination of the liver all suggested that increased Kupffer cell activity was the primary event in RES hyperphagocytosis by STZ-diabetic rats. Both hypertrophy and hyperplasia of Kupffer cells were apparent in livers of STZ-diabetic animals as evidenced by photomicrographs and hepatic cell quantification. Plasma fibronectin, which binds fibrin monomers to RE macrophages before phagocytosis, was significantly decreased in the circulation of STZ-diabetic rats, but the level of cell-associated fibronectin was not measured. Renal localization of urea-soluble /sup 125/I-fibrin monomers exceeded splenic and pulmonary uptake in normal control rats and was enhanced in animals with STZ-diabetes. Changes in fibronectin levels, fibrin monomer localization, and Kupffer cell size and numbers in experimental diabetes in rats may have implications for the pathogenesis of vascular disease involving phagocytic mesangial and foam cells in diabetic humans.
- Research Organization:
- Division of Science, Northeast Missouri State University, Kirksville
- OSTI ID:
- 6221376
- Journal Information:
- Diabetes; (United States), Journal Name: Diabetes; (United States) Vol. 31:2; ISSN DIAEA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
COAGULANTS
COLLOIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
DISPERSIONS
DRUGS
DYNAMIC FUNCTION STUDIES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE DISEASES
FIBRIN
GLANDS
HEMATOLOGIC AGENTS
HEMOSTATICS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIVER
MACROPHAGES
MAMMALS
METABOLIC DISEASES
NITROSO COMPOUNDS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PERFUSED TISSUES
PHAGOCYTES
PHAGOCYTOSIS
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
RATS
RETICULOENDOTHELIAL SYSTEM
RODENTS
SACCHARIDES
SCLEROPROTEINS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES