Estrogen influences dolichyl phosphate distribution among glycolipid pools in mouse uteri

Journal Article · Tue Mar 24 04:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00380a017· OSTI ID:6220760

Carson, D D; Tang, J; Hu, G

To determine the role that dolichyl phosphate availability plays in this induction, the authors studied the effects of estrogen priming on the content of dolichyl phosphate and the distribution of dolichyl phosphate among various glycolipids in uteri. Dolichol-linked saccharides were metabolically labeled to equilibrium with either (/sup 3/H)glucosamine or (/sup 3/H)mannose and extracted from primary explants of uterine tissue. The amount of dolichol-linked saccharide was calculated from the specific radioactivity determined for the corresponding sugar nucleotides extracted from the tissues. The major dolichol-linked saccharides identified were mannosylphosphoryldolichol (MPD), oligosaccharylpyrophosphorydolichol (OSL), and N,N'-diacetylchitobiosylpyrophosphoryldolichol (CBL). Estrogen increased the levels of MPD and OSL 4-fold; however, CBL levels did not change. After 3 days of treatment, the levels of these glycolipids were very similar to those in uteri from pregnant mice. The specific activity of GPD synthase was similar under all conditions studied. These studies provide the first determination of the levels of dolichol-linked saccharides in tissues and how these levels change during hormonal induction of glycoprotein assembly. Coupled with earlier studies, the present work demonstrates that among a number of key points of N-linked oligosaccharide assembly and transfer only synthesis of MPD increases coordinately with the increase observed in lipid- and protein-linked oligosaccharide assembly that occurs in vivo in response to estrogen. They suggest that control of MPD levels is an important regulatory aspect of N-linked glycoprotein assembly in this system.

• Research Organization: Univ. of Texas M.D. Anderson Hospital and Tumor Institute, Houston
• OSTI ID: 6220760
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:6; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
AMINES
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
ESTRADIOL
ESTRANES
ESTROGENS
FEMALE GENITALS
GLUCOPROTEINS
GLUCOSAMINE
GLUCOSE
HEXOSAMINES
HEXOSES
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLED POOL TECHNIQUES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MICE
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
STEROID HORMONES
STEROIDS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UTERUS
VERTEBRATES