Identification of subunits of acetylcholine receptor that interact with a cholesterol photoaffinity probe

Journal Article · Mon Mar 09 23:00:00 EST 1987 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00379a003· OSTI ID:6220717

Middlemas, D S; Raftery, M A

All four subunits of the acetylcholine receptor in membrane vesicles isolated from Torpedo californica have been labeled with (/sup 3/H)cholesteryl diazoacetate. As this probe incorporates into lipid bilayers analogously to cholesterol, this result indicates that acetylcholine receptor interacts with cholesterol. This investigation also demonstrates that this probe is a useful reagent for studying the interaction of cholesterol with membrane proteins.

• Research Organization: California Institute of Technology, Pasadena
• OSTI ID: 6220717
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:5; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZO COMPOUNDS
CARBOXYLIC ACID SALTS
CHEMICAL REACTIONS
CHOLESTEROL
CONFIGURATION INTERACTION
DECOMPOSITION
DRUGS
ELECTROPHORESIS
ESTERS
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARASYMPATHOMIMETICS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PROTEINS
QUATERNARY COMPOUNDS
RECEPTORS
STEROIDS
STEROLS
TRITIUM COMPOUNDS