Ozone-induced changes in the expression of the genes encoding regulatory enzymes for polyamine, ethylene and phenylpropanoid metabolisms in ozone tolerant and sensitive birch (Betula pendula Roth) clones

Conference · Sat May 01 00:00:00 EDT 1993 · Plant Physiology; (United States)

OSTI ID:6218614

Talvinen, J; Pellinen, R; Eloranta, T; Kangasjaervi, J ^[1]; Julkunen-Tiitto, R ^[2]; Karjalainen, R ^[3]

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1. Univ. of Kuopio (Finland)
2. Univ. of Joensuu (Finland)
3. Univ. of Helsinki (Finland)

Increase in the atmospheric ozone concentration has been shown to affect plant growth in several ways; from decreased photosynthetic activity to visible dames and in some extreme situations even to tissue death. Plants protect themselves from the damaging effect of ozone by inducing several physiological reactions. For example, increases in ethylene production, polyamine and phenylpropanoid synthesis have been observed in stress reaction induced by increased atmospheric ozone. similar changes, which are often called general stress reactions, are induced by several other biotic and which are often called general stress reactions, are induced by several other biontic and abiotic factors, e.g., plant pathogens. It has been shown previously that the production of stress ethylene can partly be responsible for the ozone damage formation in plants. Induction of stress-polyamine synthesis can prevent ethylene formation and is higher in some ozone-tolerant plants. We have exposed ozone sensitive and resistant birch clones to ozone (150 ppb. 8 hours) to analyze ozone-induced changes in the phenylpropanoid and polyamine metabolisms and gene expression. The polyamine and phenylpropanoid contents of the experimental material are currently being analyzed and the results will be presented. We have also cloned by PCR gene probes for birch ACC-synthase, arginine decarboxylase (ADC) and phenylalanine ammoniumlyase (PAL) genes. Results will be presented where the probes have been used to analyze ozone-induced expression of the genes in the birch clones.

• OSTI ID: 6218614
• Report Number(s): CONF-9307119-; CODEN: PLPHAY
• Journal Information: Plant Physiology; (United States), Vol. 102:1; Conference: 1993 joint annual meeting of the American Society of Plant Physiologists (ASPP) and the Canadian Society of Plant Physiologists (CSPP), Minneapolis, MN (United States), 31 Jul - 4 Aug 1993; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BIRCHES
BIOLOGICAL EFFECTS
GENETIC CONTROL
METABOLISM
OZONE
BIOLOGICAL ADAPTATION
ENZYMES
ETHYLENE
GENES
PLANT GROWTH
PLANTS
ALKENES
CONTROL
GROWTH
HYDROCARBONS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PEST CONTROL
PROTEINS
TREES
560300* - Chemicals Metabolism & Toxicology
550400 - Genetics