Detection of Listeria monocytogenes by using the polymerase chain reaction
Journal Article
·
· Applied and Environmental Microbiology; (USA)
OSTI ID:6217465
- Univ. of Colorado School of Medicine, Denver (USA)
A method was developed for detection of Listeria monocytogens by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with {sup 32}P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
- OSTI ID:
- 6217465
- Journal Information:
- Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:9; ISSN 0099-2240; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA POLYMERASES
ELECTROPHORESIS
ENZYMES
GENE AMPLIFICATION
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PATHOGENS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
TRANSFERASES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOASSAY
DAYS LIVING RADIOISOTOPES
DNA HYBRIDIZATION
DNA POLYMERASES
ELECTROPHORESIS
ENZYMES
GENE AMPLIFICATION
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PATHOGENS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIOISOTOPES
TRANSFERASES