High-performance capillary electrophoresis of histones

Conference · Tue Jan 01 04:00:00 EST 1991

DOI:https://doi.org/10.1016/0021-9673(91)80091-T· OSTI ID:6215680

Gurley, L R; London, J E; Valdez, J G

A high performance capillary electrophoresis (HPCE) system has been developed for the fractionation of histones. This system involves electroinjection of the sample and electrophoresis in a 0.1M phosphate buffer at pH 2.5 in a 50 {mu}m {times} 35 cm coated capillary. Electrophoresis was accomplished in 9 minutes separating a whole histone preparation into its components in the following order of decreasing mobility; (MHP) H3, H1 (major variant), H1 (minor variant), (LHP) H3, (MHP) H2A (major variant), (LHP) H2A, H4, H2B, (MHP) H2A (minor variant) where MHP is the more hydrophobic component and LHP is the less hydrophobic component. This order of separation is very different from that found in acid-urea polyacrylamide gel electrophoresis and in reversed-phase HPLC and, thus, brings the histone biochemist a new dimension for the qualitative analysis of histone samples. 27 refs., 8 figs.

• Research Organization: Los Alamos National Lab., NM (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6215680
• Report Number(s): LA-UR-91-510; CONF-910293--1; ON: DE91008585
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105 -- Separation Procedures
400400 -- Electrochemistry
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CAPILLARY FLOW
CHEMICAL ANALYSIS
CHO CELLS
CHROMATOGRAPHY
DESIGN
ELECTROPHORESIS
FLUID FLOW
FRACTIONATION
HISTONES
LIQUID COLUMN CHROMATOGRAPHY
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PEPTIDES
PH VALUE
PROTEINS
QUALITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
SPECTRA
ULTRAVIOLET SPECTRA