Identification of the two rotavirus genes determining neutralization specificities

Journal Article · Wed Jan 01 00:00:00 EST 1986 · J. Virol.; (United States)

OSTI ID:6207519

Offit, P A; Blavat, G

Bovine rotavirus NCDV and simian rotavirus SA-11 represent two distinct rotavirus serotypes. A genetic approach was used to determine which viral gene segments segregated with serotype-specific viral neutralization. There were 16 reassortant rotarviruses derived by coinfection of MA-104 cells in vitro with the SA-11 and NCDV strains. The parental origin of reassortant rotavirus double-stranded RNA segments was determined by gene segment mobility in polyacrylamide gels and by hybridization with radioactively labeled parental viral transcripts. The authors found that two rotavirus gene segments found previously to code for outer capsid proteins vp3 and vp7 cosegreated with virus neutralization specificities.

• Research Organization: Children's Hospital of Philadelphia, PA (USA)
• OSTI ID: 6207519
• Journal Information: J. Virol.; (United States), Vol. 57:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
VIRAL DISEASES
PATHOGENESIS
VIRUSES
HYBRIDIZATION
CATTLE
ELECTROPHORESIS
GENES
MONKEYS
PHOSPHORUS 32
RNA
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DOMESTIC ANIMALS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RUMINANTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques