Synthesis of double-stranded RNA in a virus-enriched fraction from Agaricus bisporus
Partially purified virus preparations from sporophores of Agaricus bisporus affected with LaFrance disease had up to a 15-fold-higher RNA-dependent RNA polymerase activity than did comparable preparations from health sporophores. Enzyme activity was dependent upon the presence of Mg/sup 2 +/ and the four nucleoside triphosphates and was insensitive to actinomycin D, ..cap alpha..-amanitin, and rifampin. The /sup 3/H-labeled enzyme reaction products were double-stranded RNA (dsRNA) as indicated by CF-11 cellulose column chromatography and by their ionic-strength-dependent sensitivity to hydrolysis by RNase A. The principal dsRNA products had estimated molecular weights of 4.3 /times/ 10/sup 6/ and 1.4 /times/ 10/sup 6/. Cs/sub 2/SO/sub 4/ equilibrium centrifugation of the virus preparation resolved a single peak of RNA polymerase activity that banded with a 35-nm spherical virus particle containing dsRNAs with molecular weights of 4.3 /times/ 10/sup 6/ and 1.4 /times/ 10/sup 6/. The data suggest that the RNA-dependent RNA polymerase associated with the 35-nm spherical virus is a replicase which catalyzes the synthesis of the genomic dsRNAs.
- Research Organization:
- Pennsylvania State Univ., University Park (USA)
- OSTI ID:
- 6204237
- Journal Information:
- J. Virol.; (United States), Vol. 57:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MUSHROOMS
ENZYME ACTIVITY
RNA
BIOSYNTHESIS
RNA POLYMERASES
BIOCHEMISTRY
CATIONS
MAGNESIUM COMPOUNDS
MOLECULAR WEIGHT
TRITIUM COMPOUNDS
VIRAL DISEASES
VIRUSES
ALKALINE EARTH METAL COMPOUNDS
CHARGED PARTICLES
CHEMISTRY
DISEASES
ENZYMES
FUNGI
INFECTIOUS DISEASES
IONS
LABELLED COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
SYNTHESIS
TRANSFERASES
550701* - Microbiology- Tracer Techniques