''Normal'' tissues from humans exposed to radium contain an alteration in the c-mos locus

Conference · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6202653

Huberman, E; Schlenker, R A; Hardwick, J P

The structures of a number of human proto-oncogenes from persons with internal systemic exposure to radium were analyzed by restriction enzyme digestion and southern blotting of their DNA. Two extra c-mos Eco R1 restriction-fragment-length bands of 5.0 kb and 5.5 kb were found in tissue DNA from six of seven individuals. The extra c-mos bands were detected in DNA from many, but not all, of the tissues of the individuals exposed to radium. Our results suggest that the c-mos restriction-fragment-length alterations (RFLA) found in individuals exposed to radium were induced rather than inherited, are epigenetic in origin, and most likely result from changes in the methylation of bases surrounding the single exon of the c-mos proto-oncogene. 7 refs., 3 figs., 2 tabs.

• Research Organization: Argonne National Lab., IL (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6202653
• Report Number(s): CONF-8904203-2; ON: DE89013196
• Country of Publication: United States
• Language: English

Related Subjects

560160* -- Radionuclide Effects
Kinetics
& Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADDUCTS
ALKALINE EARTH METALS
BIOLOGICAL EFFECTS
DIGESTION
DNA
DNA ADDUCTS
ELEMENTS
EPIDEMIOLOGY
GENES
GENETIC EFFECTS
INTERNAL IRRADIATION
IRRADIATION
METALS
MODIFICATIONS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
OCCUPATIONAL EXPOSURE
ONCOGENES
ORGANIC COMPOUNDS
RADIONUCLIDE KINETICS
RADIUM