Phorbol esters inhibit ammoniagenesis and gluconeogenesis in proximal tubular segments
To characterize the regulation of ammoniagenesis and gluconeogenesis in renal proximal tubule, ammonia and glucose productions were measured in suspension of canine proximal tubular segments incubated with 10 mM L-glutamine. Productions were linear functions of time for 120 min and were decreased as extracellular pH was increased from 7.0 to 7.5. To ascertain whether activation of protein kinase c affects either process, the authors incubated segments with tumor-promoting phorbol esters, 12-O-tetradecanoylphorbol-13-acetate (TPA), or phorbol 12,13-dibutyrate, or with the inactive phorbol ester 4..cap alpha..-phorbol. Ammoniagenesis and gluconeogenesis were inhibited by incubation with 10/sup /minus/6/M of the two former compounds but not the latter compound. Phorbol ester-induced inhibition was observed under conditions such that extracellular (Na/sup +/) was greater than intracellular (Na/sup +/), but not when extracellular (Na/sup +/) equaled intracellular (Na/sup +/), and was not observed in the presence of amiloride. These findings are consistent with a role for protein kinase c in the control of ammoniagenesis and gluconeogenesis in proximal tubule. Such control could be mediated via stimulation of Na/sup 1/-H/sup +/ exchange.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO (USA)
- OSTI ID:
- 6201295
- Journal Information:
- Am. J. Physiol.: Heart Circ. Physiol.; (United States), Journal Name: Am. J. Physiol.: Heart Circ. Physiol.; (United States) Vol. 21:6; ISSN AJPPD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALDEHYDES
ALKALI METAL COMPOUNDS
AMIDES
AMINO ACIDS
AMMONIA
ANIMALS
BARYONS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARCINOGENS
CATIONS
CHARGED PARTICLES
CHEMISTRY
DMSO
DOGS
ELEMENTARY PARTICLES
ENZYME INDUCTION
ENZYMES
ESTERS
FERMIONS
GENE REGULATION
GLUCOSE
GLUTAMINE
HADRONS
HEXOSES
HYDRIDES
HYDROGEN COMPOUNDS
IONS
KIDNEYS
MAMMALS
METABOLISM
MONOSACCHARIDES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PH VALUE
PHORBOL ESTERS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PHYSIOLOGY
PROTONS
SACCHARIDES
SODIUM COMPOUNDS
SULFOXIDES
TIME DEPENDENCE
TRANSFERASES
TUBULES
VERTEBRATES