Biotechnology and genetic optimization of fast-growing hardwoods

Technical Report · Fri Feb 01 04:00:00 EST 1991

DOI:https://doi.org/10.2172/6199269· OSTI ID:6199269

Garton, S; Syrkin-Wurtele, E; Griffiths, H; Schell, J; Van Camp, L; Bulka, K ^[1]

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1. NPI, Salt Lake City, UT (United States)

A biotechnology research program was initiated to develop new clones of fast-growing Populus clones resistant to the herbicide glyphosate and resistant to the leaf-spot and canker disease caused by the fungus Septoria musiva. Glyphosate-resistant callus was selected from stem segments cultured in vitro on media supplemented with the herbicide. Plants were regenerated from the glyphosate-resistant callus tissue. A portion of plants reverted to a glyphosate susceptible phenotype during organogenesis. A biologically active filtrate was prepared from S. musiva and influenced fresh weight of Populus callus tissue. Disease-resistant plants were produced through somaclonal variation when shoots developed on stem internodes cultured in vitro. Plantlets were screened for disease symptoms after spraying with a suspension of fungal spores. A frequency of 0.83 percent variant production was observed. Genetically engineered plants were produced after treatment of plant tissue with Agrobacterium tumefasciens strains carrying plasmid genes for antibiotic resistance. Transformers were selected on media enriched with the antibiotic, kanamycin. Presence of foreign DNA was confirmed by Southern blot analysis. Protoplasts of popular were produced but did not regenerate into plant organs. 145 refs., 12 figs., 36 tabs.

• Research Organization: New York State Energy Research and Development Authority, New York, NY (United States); Gas Research Inst., Chicago, IL (United States); New York Gas Group, NY (United States); NPI, Salt Lake City, UT (United States)
• Sponsoring Organization: NYSERDA; New York State Energy Research and Development Authority, Albany, NY (United States)
• OSTI ID: 6199269
• Report Number(s): NYSERDA-91-8; ON: TI92002650
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090800 -- Biomass Fuels-- Production-- (1990-)
550200 -- Biochemistry
550400 -- Genetics
553000* -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AGAR
BIOLOGICAL ADAPTATION
BIOLOGICAL VARIABILITY
BIOTECHNOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CELL CULTURES
CELL TRANSFORMATIONS
CLONE CELLS
CLONING
COLLOIDS
CULTIVATION TECHNIQUES
DISEASE RESISTANCE
DISEASES
DISPERSIONS
DNA HYBRIDIZATION
DNA-CLONING
DOCUMENT TYPES
FUNGAL DISEASES
GENETIC ENGINEERING
GENETIC VARIABILITY
GROWTH
HERBICIDES
HYBRIDIZATION
INFECTIOUS DISEASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ONTOGENESIS
ORGANIC COMPOUNDS
PESTICIDES
PLANT CELLS
PLANT GROWTH
PLANT GROWTH REGULATORS
PLANTS
PLASMIDS
POLYSACCHARIDES
POPLARS
PROGRESS REPORT
SACCHARIDES
TOLERANCE
TREES