Gene expression of pigment-binding proteins of the bacterial photosynthetic apparatus: transcription and assembly in the membrane of Rhodopseudomonas capsulata
Lowering of oxygen partial pressure in chemotrophic cultures or reduction of light intensity in phototrophic cultures of Rhodopseudomonas capsulata induced formation of the photosynthetic apparatus. A maximum of mRNA coding for the reaction center (RC) and the light-harvesting I B870 antenna complex polypeptides occurred 30 min after induction. Maximal expression of mRNA for B800-B850 antenna proteins appeared with a lag time of about 25 min after RC/B870 mRNA. Pigment-binding polypeptides were inserted into the membrane immediately after mRNA synthesis. It is concluded that the delayed formation of the B800-B850 complex compared to the RC and the B870 complex is caused by sequential expression of the corresponding genes. Biological activity of pigment-protein complexes increased after the incorporation of their polypeptides parallel to the maximum of bacteriochlorophyll synthesis. Studies on mutant strains defective in the formation of pigment-protein complexes suggested that pigment synthesis is of importance for assembly of stable complexes.
- Research Organization:
- Albert-Ludwigs-Univ., Freiburg, West Germany
- OSTI ID:
- 6194814
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:19
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PHOTOCHEMICAL REACTIONS
BIOCHEMICAL REACTION KINETICS
RHODOPSEUDOMONAS
CELL MEMBRANES
CHLOROPHYLL
GENES
MESSENGER-RNA
OXYGEN
PHOTOSYNTHESIS
POLYPEPTIDES
TRANSCRIPTION
VISIBLE RADIATION
BACTERIA
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MEMBRANES
MICROORGANISMS
NONMETALS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
RADIATIONS
REACTION KINETICS
RNA
SYNTHESIS
550200* - Biochemistry
550400 - Genetics