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Distinct local environments of the pyrene chromophores in the covalent deoxyribonucleic acid adducts of 9,10-Epoxy-9,10,11,12-tetrahydrobenzo(e)pyrene and 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene elucidated by optically detected magnetic resonance

Journal Article · · Biochemistry; (United States)
OSTI ID:6192328
;
The optically detected magnetic resonance (ODMR) spectra of the covalent DNA adduct of 9,10-epoxy-9,10,11,12-tetrahydrobenzo(e)pyrene (BePE) reveal that the excited triplet state chromophore is perturbed by the nucleic acid and that this perturbation is diminished successively by denaturation and enzymatic hydrolysis of the modified DNA, indicating that the adduct resides in an environment with some quasi-intercalative character. In contrast the covalent adduct of 7,8-dihydroxy-9,10,-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene shows little ODMR evidence of interaction with the nucleic acid, which, in view of ODMR's sensitivity demonstrated for the BePE adduct, suggests that the chromophore is situated in an environment resembling the bulk solvent. These results demonstrate phosphorescence techniques to interactions between these pyrene-like chromophores and DNA.
Research Organization:
New York Univ., New York
OSTI ID:
6192328
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 21; ISSN BICHA
Country of Publication:
United States
Language:
English

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Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
AROMATICS
BENZOPYRENE
BINDING ENERGY
CARCINOGENS
CHEMICAL REACTIONS
CLATHRATES
CONDENSED AROMATICS
COVALENCE
DECOMPOSITION
DNA
ENERGY
ENZYMATIC HYDROLYSIS
EPOXIDES
HYDROCARBONS
HYDROLYSIS
LYSIS
MAGNETIC RESONANCE
NUCLEIC ACID DENATURATION
NUCLEIC ACIDS
OPTICAL PROPERTIES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHYSICAL PROPERTIES
RESONANCE
SENSITIVITY
SOLVOLYSIS
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS