Effects of alpha-adrenergic stimulation on the regulation of the pyruvate dehydrogenase complex in the perfused rat liver
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:6181862
The regulation of the pyruvate dehydrogenase multienzyme complex was investigated during alpha-adrenergic stimulation with phenylephrine in the isolated perfused rat liver. The metabolic flux through the pyruvate dehydrogenase reaction was monitored by measuring the production of UCO2 from infused (1- UC) pyruvate. In livers from fed animals perfused with a low concentration of pyruvate (0.05 mM), phenylephrine infusion significantly inhibited the rate of pyruvate decarboxylation without affecting the amount of pyruvate dehydrogenase in its active form. Results show that alpha-adrenergic agonists do not exert short term regulatory effects on pyruvate dehydrogenase in the liver. Furthermore, the results suggest either that the rat liver pyruvate dehydrogenase complex is insensitive to changes in mitochondrial calcium or that changes in intramitochondrial calcium levels as a result of alpha-adrenergic stimulation are considerably less than suggested by others.
- Research Organization:
- Univ. of Texas Health Science Center, San Antonio
- OSTI ID:
- 6181862
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 16; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
DECARBOXYLATION
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
GLANDS
HEMIACETAL DEHYDROGENASES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KETO ACIDS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PYRUVIC ACID
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOCHEMICAL REACTION KINETICS
BODY
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
DECARBOXYLATION
DIGESTIVE SYSTEM
DRUGS
ENZYME ACTIVITY
ENZYMES
GLANDS
HEMIACETAL DEHYDROGENASES
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
KETO ACIDS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PYRUVIC ACID
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SYMPATHOMIMETICS
TRACER TECHNIQUES
VERTEBRATES