Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells

Journal Article · Mon Apr 01 00:00:00 EST 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.82.8.2399· OSTI ID:6181644

Sutherland, B M; Bennett, P B; Freeman, A G; Moore, S P; Strickland, P T

Neonatal human foreskin fibroblasts can be transformed to anchorage-independent growth by transfection with DNAs inefficient in transforming NIH 3T3 cells. Human cells transfected with DNA from GM 1312, a multiple myeloma cell line, or MOLT-4, a permanent lymphoblast line, grow without anchorage at a much higher frequency than do the parental cells and their DNAs can transform human cell recipients to anchorage-independent growth; they have extended but not indefinite life spans and are nontumorigenic. Human fibroblasts are also transformed by DNAs from two multiple myeloma lines that also transform 3T3 cells; however, restriction analysis suggests that different transforming genes in this DNA are acting in the human and murine systems. These results indicate that the human cell transfection system allows detection of transforming genes not effective in the 3T3 system and points out the possibility of detection of additional transforming sequences even in DNAs that do transform murine cells.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States)
• OSTI ID: 6181644
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 82:8
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
ONCOGENIC TRANSFORMATIONS
TUMOR CELLS
DNA
CARCINOGENESIS
CYTOLOGICAL TECHNIQUES
INFANTS
MICE
PLASMIDS
SKIN
AGE GROUPS
ANIMAL CELLS
ANIMALS
BODY
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CHILDREN
CONNECTIVE TISSUE CELLS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
RODENTS
SOMATIC CELLS
VERTEBRATES
550300* - Cytology