Radioreceptor assay for epidermal growth factor. [/sup 125/I tracer technique, mice]

Journal Article · Thu Mar 01 04:00:00 EST 1979 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/S0003-2697(79)80153-0· OSTI ID:6180712

Ladda, R L; Bullock, L P; Gianopoulos, T; McCormick, L

An established cell line of human lung fibroblasts with a high number of surface receptors for mouse epidermal growth factor (mEGF) was used to deveop a simple and highly sensitive radioreceptor assay for EGF. /sup 125/I-Labeled mEGF competed mole for mole with unlabeled mEGF for specific receptors. Optimal range for discriminating EGF concentrations in body fluids and tissue extracts by a competitive binding assay was between 5 and 100 ng/ml. Interassay correlation of variation was 8.47% and the recovery of highly purified mEGF added to serum and urine samples was greater than 95%. Human serum and amniotic fluids contained about 24 and 4 ng/ml, respectively, of mEGF equivalents. Concentrations of mEGF in mouse urine and serum were highly variable and were 2- to 10-fold greater than that previously detected by radioimmune assay. Hypophysectomy nearly abolished submaxillary mEGF content in both male and female mice, but testosterone treatment of hypophysectomized animals restored normal concentrations of mEGF to the glands. mEGF added to culture medium disappeared with time as a function of the number of cellular EGF receptors indicating cellular degradation of the growth factor. The radioreceptor assay for EGF is based on the close biologic relationship between the cell receptor site and the native hormone and should prove to be a useful complementary tool to characterize the physiological role of EGF.

• Research Organization: Pennsylvania State Univ., Hershey
• OSTI ID: 6180712
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 93:2; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
FIBROBLASTS
GROWTH
HORMONES
HYDROXY COMPOUNDS
HYPOPHYSECTOMY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KETONES
LUNGS
MAMMALS
MEDICINE
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOISOTOPES
RECEPTORS
RESPIRATORY SYSTEM
RODENTS
SENSITIVITY
SKIN
SOMATIC CELLS
STEROID HORMONES
STEROIDS
STH
SURGERY
TESTOSTERONE
TISSUES
VERTEBRATES