Selective regulation of intrinsic membrane proteins in HepG2

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6180162

Collins, J C; Wolkoff, A W; Morell, A G; Stockert, R J

The asialoglycoprotein receptor (ASGP-R) and insulin receptor (IR) are cell-surface glycoproteins whose activity varies with the state of cellular differentiation. Expression of ASGP-R, IR and an organic anion binding protein (OABP) was studied in HepG2 cells grown in minimal essential medium (MEM) supplemented with 10% fetal bovine serum (FBS), dialyzed FBS (dFBS) or serum from other species and corresponding to different physiological states of the liver. Cells grown in MEM-10% FBS maximally express ASGP-R and IR binding activity at confluence while OABP antigen appears independent of the state of cellular proliferation. Growth in dFBS reduces expression of ASGP-R and IR at confluence by 60-80% without altering OABP content, total cellular protein synthesis, or /sup 3/H-thymidine incorporation. Immunoblot of these cells reveals virtually no mature, 45 kDa ASGP-R, but a small amount of 36 kDa protein; metabolic labelling with /sup 35/S-methionine shows reduced synthesis of 45 kDa ASGP-R and absence of the 36 kDa antigen. Northern blot analysis of ASGP-R mRNA levels suggests post-translational regulation is affected. Normal expression of ASGP-R and IR are restored by addition of a 300-350 dalton fraction of FBS to medium.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY
• OSTI ID: 6180162
• Report Number(s): CONF-870644-; TRN: 87-033986
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
INSULIN
LIVER CELLS
CELL DIFFERENTIATION
CELL MEMBRANES
METHIONINE
PROTEINS
SULFUR 35
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DRUGS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
RIBOSIDES
SACCHARIDES
SOMATIC CELLS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques