Hypersensitive prostaglandin and thromboxane response to hormones in rabbit colitis

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Am. J. Physiol.; (United States)

OSTI ID:6179574

Zipser, R D; Patterson, J B; Kao, H W; Hauser, C J; Locke, R

Inflammation of the colon is associated with increased production of prostaglandins (PG) and thromboxanes (Tx), and these eicosanoids may contribute to the inflammatory, secretory, and motility dysfunctions in colitis. To evaluate the potential role of peptide hormones in the enhanced eicosanoid release, colitis was established in rabbits by a delayed-type hypersensitivity reaction to dinitrochlorobenzene and by an immune-complex-mediated reaction. PG and Tx were identified in the venous effluent of isolated perfused colons by radiochromatography after ( UC)arachidonic acid prelabeling, as well as by bioassay, and then quantitated by immunoassay. The two colitis models were morphologically similar. Basal release of PGE2, PGI2, and TxA2 was two- to threefold greater from colitis tissue than from control tissue. Bradykinin (BK) and angiotensin II (ANG II) increased release of UC-labeled eicosanoids, whereas several gastrointestinal hormones had no effect. In control colons, BK and ANG II increased PGE2 and PGI2 release (by about 2-fold) but did not alter TxA2. In contrast, BK and ANG II markedly exaggerated the release of eicosanoids in colitis. Since BK and possibly ANG II are increased at sites of inflammation, the hypersensitive eicosanoid response to these peptides may augment the eicosanoid-mediated manifestations of colitis.

• OSTI ID: 6179574
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

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551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CARBON 14 COMPOUNDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
HORMONES
IMMUNE REACTIONS
IMMUNOLOGY
INFLAMMATION
INTESTINES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
ORGANS
PATHOLOGICAL CHANGES
PEPTIDE HORMONES
PROSTAGLANDINS
RABBITS
RADIOASSAY
RADIOCHROMATOGRAPHY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
SYMPTOMS
TRACER TECHNIQUES
VERTEBRATES