Synthetic peptides used to locate the. cap alpha. -bungarotoxin binding site and immunogenic regions on. cap alpha. subunits of the nicotinic acetylcholine receptor
Synthetic peptides corresponding to 57% of the sequence of ..cap alpha.. subunits of acetylcholine receptors from Torpedo californica electric organ and extending from the NH/sub 2/ to the COOCH terminus have been synthesized. The ..cap alpha..-bungarotoxin binding site on denatured ..cap alpha.. subunits was mapped within the sequence ..cap alpha..185-199 by assaying binding of /sup 125/I-..cap alpha..-bungarotoxin to slot blots of synthetic peptides. Further studies showed that residues in the sequence ..cap alpha..190-194, especially cysteines-..cap alpha..192,193, were critical for binding ..cap alpha..-bungarotoxin. Reduction and alkylation studies suggested that these cysteines must be disulfide linked for ..cap alpha..-bungarotoxin to bind. Binding sites for serum antibodies to native receptors or ..cap alpha.. subunits were mapped by indirect immunoprecipitation of /sup 125/I-peptides. Several antigenic sequences were identified, but a synthetic peptide corresponding to the main immunogenic region (which is highly conformation dependent) was not identified.
- Research Organization:
- Salk Institute for Biological Studies, San Diego, CA
- OSTI ID:
- 6178312
- Journal Information:
- Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 26:12; ISSN BICHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETYLCHOLINE
AMINES
AMINO ACID SEQUENCE
AMMONIUM COMPOUNDS
ANTIBODIES
ANTIGENS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MATERIALS
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOMIMETICS
PEPTIDES
PROTEINS
QUATERNARY COMPOUNDS
RADIOCHROMATOGRAPHY
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
SEPARATION PROCESSES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES