delta-crystallin genes become hypomethylated in postmitotic lens cells during chicken development
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
Although it has been argued that the loss of 5-methylcytosine from specific sites in DNA plays an important role in activation of specific genes, the mechanism of hypomethylation is not well understood. One model links the process to DNA replication, proposing that it occurs by not remethylating cytosine on newly synthesized DNA. An alternative model argues that hypomethylation results from excision of part or all of the 5-methylcytosine. The authors were able to test whether hypomethylation can occur without replication by analysis of methylation changes in the delta-crystallin genes of the chicken lens. During embryonic development a large fraction of cells in the lens stops dividing as part of the differentiation process, measured by autoradiography. Shortly after this stage, the delta-crystallin genes in samples of the whole lens become hypomethylated, suggesting the possibility that this process might be occurring in the subset of cells that is no longer dividing. They found that hypomethylation of these genes does occur in postmitotic lens cells, a result that implicates an excision mechanism in this tissue.
- Research Organization:
- Univ. of Virginia, Charlottesville
- OSTI ID:
- 6178067
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:2; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
AZINES
BIRDS
BODY
BODY AREAS
CHEMICAL REACTIONS
CHICKENS
CRYSTALLINE LENS
DNA REPLICATION
EMBRYOS
EYES
FACE
FOWL
GENE REGULATION
GENES
HEAD
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
METHYLATION
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
SENSE ORGANS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AUTORADIOGRAPHY
AZINES
BIRDS
BODY
BODY AREAS
CHEMICAL REACTIONS
CHICKENS
CRYSTALLINE LENS
DNA REPLICATION
EMBRYOS
EYES
FACE
FOWL
GENE REGULATION
GENES
HEAD
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
METHYLATION
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ONTOGENESIS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RIBOSIDES
SENSE ORGANS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES