Fatty acyl specificity of the receptor-mediated release of polyunsaturated fatty acids from vascular endothelial cells
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6173173
Histamine and bradykinin appear to exhibit the same fatty acid specificity as thrombin. Incubation of human umbilical vein endothelial cells with 10 ..mu..M histamine for 10 min in buffered saline containing 50 ..mu..M fat-free albumin stimulates the release of previously incorporated (/sup 14/C)arachidonate but not (/sup 14/C)22:4(n-6) or (/sup 14/C)20:3(n-6). Similarly calf pulmonary artery endothelial cells release (/sup 14/C)arachidonate but not (/sup 14/C)22:4(n-6) in response to either bradykinin (1 /sup +/g/ml) or histamine (10..mu..M). In both types of endothelial cells, the calcium ionophore A23187 (10 ..mu..M) exhibits the same pattern of fatty acyl specificity as the receptor-mediated agonists. By contrast, mellitin (2-4 ..mu..g/ml) stimulates the release of free 22:4(n-6) and oleate in addition to arachidonate; release of 22:4(n-6) is 30-70% that of arachidonate. These results suggest that histamine, bradykinin and thrombin stimulate a common calcium-dependent fatty acyl-specific phospholipase activity.
- Research Organization:
- Eastern Virginia Medical School, Norfolk
- OSTI ID:
- 6173173
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BODY
BRADYKININ
CALVES
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATTLE
COAGULANTS
DOMESTIC ANIMALS
DRUGS
ENDOTHELIUM
ENZYME ACTIVITY
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROLASES
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LIPASE
MAMMALS
MAN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
REACTION KINETICS
RUMINANTS
SERINE PROTEINASES
SPECIFICITY
THROMBIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
AZOLES
BIOCHEMICAL REACTION KINETICS
BODY
BRADYKININ
CALVES
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CATTLE
COAGULANTS
DOMESTIC ANIMALS
DRUGS
ENDOTHELIUM
ENZYME ACTIVITY
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HISTAMINE
HYDROLASES
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
LIPASE
MAMMALS
MAN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
REACTION KINETICS
RUMINANTS
SERINE PROTEINASES
SPECIFICITY
THROMBIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES