Fatty acyl specificity of the receptor-mediated release of polyunsaturated fatty acids from vascular endothelial cells

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6173173

Rosenthal, M D

Histamine and bradykinin appear to exhibit the same fatty acid specificity as thrombin. Incubation of human umbilical vein endothelial cells with 10 ..mu..M histamine for 10 min in buffered saline containing 50 ..mu..M fat-free albumin stimulates the release of previously incorporated (/sup 14/C)arachidonate but not (/sup 14/C)22:4(n-6) or (/sup 14/C)20:3(n-6). Similarly calf pulmonary artery endothelial cells release (/sup 14/C)arachidonate but not (/sup 14/C)22:4(n-6) in response to either bradykinin (1 /sup +/g/ml) or histamine (10..mu..M). In both types of endothelial cells, the calcium ionophore A23187 (10 ..mu..M) exhibits the same pattern of fatty acyl specificity as the receptor-mediated agonists. By contrast, mellitin (2-4 ..mu..g/ml) stimulates the release of free 22:4(n-6) and oleate in addition to arachidonate; release of 22:4(n-6) is 30-70% that of arachidonate. These results suggest that histamine, bradykinin and thrombin stimulate a common calcium-dependent fatty acyl-specific phospholipase activity.

• Research Organization: Eastern Virginia Medical School, Norfolk
• OSTI ID: 6173173
• Report Number(s): CONF-870644-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BRADYKININ
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
SPECIFICITY
HISTAMINE
LIPASE
ENZYME ACTIVITY
THROMBIN
CALVES
CARBON 14 COMPOUNDS
ENDOTHELIUM
MAN
TRACER TECHNIQUES
AMINES
ANIMAL TISSUES
ANIMALS
AZOLES
BODY
CARBOXYLESTERASES
CATTLE
COAGULANTS
DOMESTIC ANIMALS
DRUGS
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROLASES
IMIDAZOLES
ISOTOPE APPLICATIONS
KINETICS
KININS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
REACTION KINETICS
RUMINANTS
SERINE PROTEINASES
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques