NMR studies of the membrane bound form of filamentous bacteriophage fd and Pfl major coat proteins
The major coat proteins of the fd (M13) and Pf1 filamentous bacteriophage exist as integral membrane proteins during the viral life cycle. These proteins adopt their membrane bound conformations when solubilized by a variety of detergents, and the protein-detergent micelle complexes can be studied using solution NMR techniques. Determination of the structure of the coat proteins in their membrane bound form has been accomplished by qualitative interpretation of 2-dimensional /sup 1/H-/sup 1/H NOE spectra (NOESY). The critical amide proton resonance assignments were made through biosynthetic /sup 15/N labeling and /sup 1/H//sup 15/N heteronuclear chemical shift correlation techniques. The data indicate that both proteins adopt helical conformations within the micelle. The /sup 15/N//sup 1/H heteronuclear NOE has been used to characterize the backbone dynamics of both proteins in micelles. The lipid associated residues of the proteins are rigid on the nanosecond timescale, while the hydrophilic solvent associated N- and C-termini are high mobile. These results complement previously reported protein dynamics studies of membrane bound coat proteins conducted using solid state NMR methods. Solid state NMR studies reported in the literature have also investigated the structure and dynamics of the fd and Pf1 major coat proteins when bound to intact phage. Therefore, structure/dynamics comparisons of the proteins in their structural versus membrane bound forms can be made.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6172893
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIOPHAGES
BARYONS
CELL CONSTITUENTS
CELL MEMBRANES
CONFIGURATION
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HELICAL CONFIGURATION
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MEMBRANES
MICROORGANISMS
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASITES
PROTEINS
PROTONS
RESONANCE
SPECTRA
STABLE ISOTOPES
VIRUSES