Guanosine 3 prime ,5 prime -cyclic nucleotide binding proteins of bovine retina identified by photoaffinity labeling

Journal Article · Thu Mar 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.6.2201· OSTI ID:6172213

Thompson, D A; Khorana, H G ^[1]

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1. Massachusetts Institute of Technology, Cambridge (USA)

Cyclic GMP-binding proteins present in membrane fractions of bovine retina and, in particular, rod outer segments (ROS) were identified by photoaffinity labeling with 8-azido-({sup 32}P)cGMP. Two soluble proteins and two membrane-associated proteins were specifically labeled. The soluble proteins, 93 and 72 kDa, corresponded respectively to the {alpha} subunit of ROS cGMP phosphodiesterase and cGMP-dependent protein kinase. One of the two membrane-associated proteins, 53 kDa, was present in all particulate retinal fractions. Its function is unknown. It is distinct from cAMP-dependent protein kinase or the 63-kDa cGMP-activated channel from ROS. The second membrane-associated protein, 37 kDa, was present only in fractions that did not contain ROS. The molecular mass of this protein was similar to that of cGMP-binding protein previously attributed to rod cells.

• OSTI ID: 6172213
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GUANOSINE
RECEPTORS
MEMBRANE PROTEINS
AUTORADIOGRAPHY
CATTLE
ELECTROPHORESIS
LABELLING
MOLECULAR STRUCTURE
PHOSPHODIESTERASES
PHOSPHORUS 32
RETINA
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ENZYMES
ESTERASES
EYES
FACE
HEAD
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
PURINES
RADIOISOTOPES
RIBOSIDES
RUMINANTS
SENSE ORGANS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques