In vitro radiolabel uptake viability assay for Onchocerca microfilariae
Journal Article
·
· J. Parasitol.; (United States)
A radiolabel uptake viability assay for Onchocerca cervicalis using (/sup 3/H)2-deoxy-D-glucose in Hanks' balanced salt solution, pH 7.5, at 30 C is described and compared to the traditional visual motility assay. A correlation of r = 0.92 between the assays was found, with the radiolabel uptake method apparently a more sensitive indicator of microfilarial viability.
- Research Organization:
- Medical Univ. of South Carolina, Charleston (USA)
- OSTI ID:
- 6172154
- Journal Information:
- J. Parasitol.; (United States), Vol. 75:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NEMATODES
VIABILITY
GLUCOSE
IN VITRO
PARASITES
PARASITIC DISEASES
RADIOASSAY
TRITIUM COMPOUNDS
ALDEHYDES
ASCHELMINTHES
CARBOHYDRATES
DISEASES
HELMINTHS
HEXOSES
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
550901* - Pathology- Tracer Techniques
NEMATODES
VIABILITY
GLUCOSE
IN VITRO
PARASITES
PARASITIC DISEASES
RADIOASSAY
TRITIUM COMPOUNDS
ALDEHYDES
ASCHELMINTHES
CARBOHYDRATES
DISEASES
HELMINTHS
HEXOSES
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
550901* - Pathology- Tracer Techniques