Differential effects of fibroblast growth factor on insulin receptor and muscle specific protein gene expression in BC3H-1 myocytes
- Mount Zion Hospital and Medical Center, San Francisco, CA (USA)
BC3H-1 mouse muscle cells in culture were employed to study the mechanisms which regulate insulin receptor gene expression during differentiation. When BC3H-1 myoblasts were plated in low serum media (1% fetal bovine serum), cell division ceased. Within 1 week cells had the morphological features of myocytes and expressed muscle specific proteins such as creatine phosphokinase and the nicotinic acetylcholine receptor. It is known that following incubation in low serum media, the steady state mRNA levels for the key muscle transcription factor, myogenin are increased. Exposure of BC3H-1 cells to a 20-base myogenin antisense oligomer blocked morphological differentiation, and resulted in nearly complete inhibition of the expression of the acetylcholine receptor but not the insulin receptor(IR). In order to study further the relationship between differentiation and IR gene expression, fibroblast growth factor (FGF), a known inhibitor of myogenic differentiation, was employed. FGF treatment inhibited the transcription of both myogenin and the acetylcholine receptor. However FGF did not inhibit the transcription of the IR. These studies indicate therefore that IR transcription increases during muscle cell differentiation, and suggest that during differentiation the control of IR gene expression differs from the control of muscle specific proteins.
- OSTI ID:
- 6171947
- Journal Information:
- Molecular Endocrinology; (USA), Vol. 4:6; ISSN 0888-8809
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
CELL DIFFERENTIATION
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
INSULIN
RECEPTORS
PROTEINS
GENE REGULATION
CELL CULTURES
CULTURE MEDIA
FIBROBLASTS
INHIBITION
IODINE ISOTOPES
MESSENGER-RNA
MICE
MUSCLES
TRACER TECHNIQUES
TRANSCRIPTION
ANIMALS
CONNECTIVE TISSUE CELLS
HORMONES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEPTIDE HORMONES
REACTION KINETICS
RNA
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques