Growth hormone releasing factor (GRF) increases free arachidonate levels in the pituitary: a role for lipoxygenase products

Journal Article · Mon Jan 20 00:00:00 EST 1986 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(86)90312-7· OSTI ID:6171370

Canonico, P L; Speciale, C; Sortino, M A; Cronin, M J; MacLeod, R M; Scapagnini, U

GRF, a specific stimulator of GH release, increased in a concentration- and time-dependent manner pituitary (/sup 3/H)-arachidonate levels in vitro. This effect was antagonized by 100 nM somatostatin. Exogenous arachidonate also stimulated GH release in vitro. Quinacrine, a phospholipase A2 inhibitor, reduced both basal and GRF-stimulated free arachidonate levels as well as GH release. The cyclooxygenase inhibitor indomethacin was ineffective, while BW755c, which also inhibits the lipoxygenase pathway, produced a further increase in the levels of the fatty acid stimulated by GRF and potently reduced GH release. These results provide additional evidence for the involvement of arachidonate metabolism in the hormone-releasing effect of GRF at the somatotroph. 14 references, 1 figure, 2 tables.

• Research Organization: Univ. of Catania School of Medicine, Italy
• OSTI ID: 6171370
• Journal Information: Life Sci.; (United States), Vol. 38:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
METABOLISM
OXYGENASES
ENZYME ACTIVITY
STH
SECRETION
CARBOXYLIC ACIDS
DOSE-RESPONSE RELATIONSHIPS
ENZYME INHIBITORS
EXPERIMENTAL DATA
IN VITRO
PITUITARY GLAND
SOMATOSTATIN
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
BODY
DATA
ENDOCRINE GLANDS
ENZYMES
GLANDS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
550201* - Biochemistry- Tracer Techniques