Growth hormone releasing factor (GRF) increases free arachidonate levels in the pituitary: a role for lipoxygenase products
Journal Article
·
· Life Sci.; (United States)
GRF, a specific stimulator of GH release, increased in a concentration- and time-dependent manner pituitary (/sup 3/H)-arachidonate levels in vitro. This effect was antagonized by 100 nM somatostatin. Exogenous arachidonate also stimulated GH release in vitro. Quinacrine, a phospholipase A2 inhibitor, reduced both basal and GRF-stimulated free arachidonate levels as well as GH release. The cyclooxygenase inhibitor indomethacin was ineffective, while BW755c, which also inhibits the lipoxygenase pathway, produced a further increase in the levels of the fatty acid stimulated by GRF and potently reduced GH release. These results provide additional evidence for the involvement of arachidonate metabolism in the hormone-releasing effect of GRF at the somatotroph. 14 references, 1 figure, 2 tables.
- Research Organization:
- Univ. of Catania School of Medicine, Italy
- OSTI ID:
- 6171370
- Journal Information:
- Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 38:3; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mechanism for release of arachidonic acid during guinea pig platelet aggregation: a role for the diacylglycerol lipase inhibitor RHC 80267
Dual effect of nitrogen dioxide on rat alveolar macrophage arachidonate metabolism
Ketoconazole attenuates radiation-induction of tumor necrosis factor
Thesis/Dissertation
·
Tue Dec 31 23:00:00 EST 1985
·
OSTI ID:6655480
Dual effect of nitrogen dioxide on rat alveolar macrophage arachidonate metabolism
Journal Article
·
· Experimental Lung Research; (United States)
·
OSTI ID:6656532
Ketoconazole attenuates radiation-induction of tumor necrosis factor
Journal Article
·
Fri Jul 01 00:00:00 EDT 1994
· International Journal of Radiation Oncology, Biology and Physics
·
OSTI ID:86487
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BODY
CARBOXYLIC ACIDS
DATA
DOSE-RESPONSE RELATIONSHIPS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
GLANDS
HORMONES
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
SECRETION
SOMATOSTATIN
STH
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BODY
CARBOXYLIC ACIDS
DATA
DOSE-RESPONSE RELATIONSHIPS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
GLANDS
HORMONES
IN VITRO
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
SECRETION
SOMATOSTATIN
STH
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS