sup 1 H and sup 31 P nuclear magnetic resonance investigation of the interaction between 2,3-diphosphoglycerate and human normal adult hemoglobin

Journal Article · Tue Apr 10 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00467a027· OSTI ID:6170335

Russu, I M; Wu, S S; Bupp, K A; Ho, N T; Ho, C ^[1]

---

1. Carnegie Mellon Univ., Pittsburgh, PA (USA)

High-resolution {sup 1}H and {sup 31}P nuclear magnetic resonance spectroscopy has been used to investigate the binding of 2,3-diphosphoglycerate to human normal adult hemoglobin and the molecular interactions involved in the allosteric effect of the 2,3-diphosphoglycerate molecule on hemoglobin. Individual hydrogen ion NMR titration curves have been obtained for 22-26 histidyl residues of hemoglobin and for each phosphate group of 2,3-diphosphoglycerate with hemoglobin in both the deoxy and carbonmonoxy forms. The results indicate that 2,3-diphosphoglycerate binds to deoxyhemoglobin at the central cavity between the two {beta} chains and the binding involves the {beta}2-histidyl residues. Moreover, the results suggest that the binding site of 2,3-diphosphoglycerate to carbonmonoxyhemoglobin contains the same (or at least some of the same) amino acid residues responsible for binding in the deoxy form. As a result of the specific interactions with 2,3-diphosphoglycerate, the {beta}2-histidyl residues make a significant contribution to the alkaline Bohr effect under these experimental conditions. These results give the first experimental demonstration that long-range electrostatic and/or conformation effects of the binding could play an important role in the allosteric effect of 2,3-diphosphoglycerate on hemoglobin. The {sup 31}P nuclear magnetic resonance titration data for each phosphate group of 2,3-diphosphoglycerate have been used to calculate the pK values of the phosphate groups in 2,3-diphosphoglycerate bound to deoxy- and carbon-monoxyhemoglobin and the proton uptake by 2,3-diphosphoglycerate upon ligand binding to hemoglobin.

• OSTI ID: 6170335
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:14; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADULTS
AGE GROUPS
AMINO ACIDS
ANIMALS
AZOLES
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
ELEMENTARY PARTICLES
ELEMENTS
ERYTHROCYTES
FERMIONS
GLOBINS
GLYCERIC ACID
HADRONS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
HYDROXY ACIDS
IMIDAZOLES
ISOTOPES
LIGANDS
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MAN
MATERIALS
NMR SPECTRA
NONMETALS
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PH VALUE
PHOSPHORUS 31
PHOSPHORUS ISOTOPES
PIGMENTS
PORPHYRINS
PRIMATES
PROTEINS
PROTONS
RESONANCE
SPECTRA
STABLE ISOTOPES
VERTEBRATES