Survival in subpopulations of cells derived from solid KHT sarcomas by centrifugal elutriation following treatment with CCNU and MISO

Journal Article · Sat Sep 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90513-3· OSTI ID:6166996

Hill, S A; Keng, P C; Siemann, D W

Misonidazole (MISO) has been shown to enhance the cytotoxicity of 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) in a number of different animal tumor systems. The authors have investigated the response to therapy of the various subpopulations of cells comprising the KHT sarcoma, to determine whether chemopotentiation occurred as a preferential enhancement of killing in one subpopulation of cells. Twenty-four hr after drug treatment, cells dissociated from solid tumors were separated into homogeneous populations based on cell size by the technique of centrifugal elutriation. Clonogenic cell survival was measured for each elutriated fraction. In vivo treatment with MISO produced no measurable cell-kill across the cell cycle. Those cells in late G/sub 1/ and S phase 24 hr after treatment were most sensitive to CCNU alone. The enhancement of CCNU cytotoxicity by MISO occurred primarily in the early G/sub 1/ and S fractions. These data suggest that chemopotentiation does not occur equally in all tumor cell subpopulations and that some specificity of enhanced cell killing exists.

• Research Organization: Univ. of Rochester Cancer Center, NY
• OSTI ID: 6166996
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:9; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
AZOLES
BIOLOGICAL EFFECTS
CELL CYCLE
CELL FLOW SYSTEMS
CELL KILLING
CENTRIFUGATION
DISEASES
DRUGS
EXPERIMENTAL NEOPLASMS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
MISONIDAZOLE
NEOPLASMS
NITROSO COMPOUNDS
NITROSOUREAS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIOSENSITIZERS
SARCOMAS
SEPARATION PROCESSES
TUMOR CELLS