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Effect of intercellular contact on the radiation sensitivity of KHT sarcoma cells

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575087· OSTI ID:6164295
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The recent finding that some cells grown in vitro as spheroids demonstrate an intercellular contact effect which increases their capacity to accumulate and repair sublethal radiation damage has important implications for the interpretation of results obtained from in vivo irradiations. The experiments reported in this paper demonstrate that the intercellular contact effect can be observed in an animal tumor system in vivo. The studies were performed with KHT sarcoma cells, irradiated in contact as small lung colonies in situ or as individual cells in vitro. The cells for the in vitro irradiations were obtained from subcutaneous tumors which were excised from their host mice, immediately made into cell suspensions, and then irradiated as a function of time after excision of the tumors from their host animals. Survival curves obtained at 2 or 24 hr after tumor excision have identical slopes (D/sub 0/ = 129 +- 7 and 121 +- 12 rad, respectively), but extrapolation numbers which differ by a factor of 7 (17 and 2.5, respectively). The cells irradiated in contact as small lung colonies have a survival curve with a shoulder at least as large as that seen at 2 hr after tumor excision. Split-dose experiments performed for the various conditions discussed indicate that the size of the shoulder of the survival curve reflects the ability of the cells to repair sublethal radiation damage.
Research Organization:
Ontario Cancer Inst., Toronto, Canada
OSTI ID:
6164295
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 77:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550300 -- Cytology
560121 -- Radiation Effects on Cells-- External Source-- (-1987)
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CELL CULTURES
CESIUM 137
CESIUM ISOTOPES
COMPARATIVE EVALUATIONS
CULTIVATION TECHNIQUES
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
FRACTIONATED IRRADIATION
GAMMA RADIATION
IN VITRO
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
LUNGS
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
RESPIRATORY SYSTEM
RODENTS
SURVIVAL CURVES
TUMOR CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
YEARS LIVING RADIOISOTOPES