High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones

Journal Article · Thu Jan 04 23:00:00 EST 1990 · Science (Washington, D.C.); (USA)

DOI:https://doi.org/10.1126/science.2294592· OSTI ID:6158909

Lichter, P; Chang Tang, C J; Ward, D C ^[1]; Call, K; Housman, D ^[2]; Hermanson, G; Evans, G A ^[3]

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1. Yale Univ. School of Medicine, New Haven, CT (USA)
2. Massachusetts Institute of Technology, Cambridge (USA)
3. Salk Institute, La Jolla, CA (USA)

Cosmid clones containing human DNA inserts have been mapped on chromosome 11 by fluorescence in situ hybridization under conditions that suppress signal from repetitive DNA sequences. Thirteen known genes, one chromosome 11-specific DNA repeat, and 36 random clones were analyzed. High-resolution mapping was facilitated by using digital imaging microscopy and by analyzing extended (prometaphase) chromosomes. The map coordinates established by in situ hybridization showed a one to one correspondence with those determined by Southern (DNA) blot analysis of hybrid cell lines containing fragments of chromosome 11. Furthermore, by hybridizing three or more cosmids simultaneously, gene order on the chromosome could be established unequivocally. These results demonstrate the feasibility of rapidly producing high-resolution maps of human chromosomes by in situ hybridization.

• OSTI ID: 6158909
• Journal Information: Science (Washington, D.C.); (USA), Journal Name: Science (Washington, D.C.); (USA) Vol. 247:4938; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FEASIBILITY STUDIES
FLUORESCENCE
GENETIC MAPPING
HYBRIDIZATION
LUMINESCENCE
MAMMALS
MAN
MAPPING
MOLECULAR BIOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES