Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Purification and characterization of a membrane-associated 3,3',5-triiodo-L-thyronine binding protein from a human carcinoma cell line

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
A membrane-associated binding protein for 3,3',5-triiodo-L-thyronine (T3) was purified to apparent homogeneity from A431 human epidermoid carcinoma cells. A431 cells were specifically labeled with the N-bromoacetyl derivative of T3 labeled with SVI at the 3' position (BrAc( SVI)T3) and were extracted with 3-(3-(cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), a zwitterionic detergent. The solubilized BrAc( SVI)T3-labeled protein was successively purified by chromatography on Sephadex G-200 and QAE-Sephadex followed by NaDodSO4/PAGE. Approximately 0.2 mg of purified protein was obtained from 2.5 x 10Z cells, which represents a 3000-fold purification. The membrane-associated T3 binding protein is an acidic protein with a pI of 5.1 and an apparent molecular mass of 55,000 daltons determined by NaDodSO4/PAGE. Polyclonal antibodies against the 55-kDa protein were prepared and used in indirect immunofluorescence to show that the 55-kDa protein was mainly found in the nuclear envelope and endoplasmic reticulum.
Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
6156008
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Journal Issue: 4 Vol. 83:4; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of the 3,3',5-triiodo-L-thyronine-binding site on plasma membranes from human placenta
Journal Article · Sat Jun 01 00:00:00 EDT 1985 · Endocrinology; (United States) · OSTI ID:5021556
Selective affinity labeling of a 27-kDa integral membrane protein in rat liver and kidney with N-bromoacetyl derivatives of L-thyroxine and 3,5,3'-triiodo-L-thyronine
Journal Article · Sun Apr 15 00:00:00 EDT 1990 · Journal of Biological Chemistry; (USA) · OSTI ID:7118071
Purification and subunit structure of the (/sup 3/H)phenamil receptor associated with the renal apical Na/sup +/ channel
Journal Article · Wed Jul 01 00:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5297815

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CARCINOMAS
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
CONFIGURATION INTERACTION
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
EVEN-ODD NUCLEI
FLUORESCENCE
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
MEMBRANES
METHIONINE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PROTEINS
PURIFICATION
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
THYROID HORMONES
TRIIODOTHYRONINE
TUMOR CELLS