Identification and characterization of an angiotensin II receptor on cultured bovine adrenal chromaffin cells

Thesis/Dissertation · Thu Jan 01 04:00:00 EST 1987

OSTI ID:6154723

Boyd, V L

The presence of an angiotensin II receptor on cultured bovine adrenal chromaffin cells was demonstrated by radioligand binding. A single class of finding sites with a K/sub D/ of 0.7 nM was characterized. The use of radioligands also allows the localization of receptors by autoradiography. Autoradiography demonstrated that approximately 50% of the isolated cells bound angiotensin II. It was of interest to see if angiotensin II bound to a cell that possessed a certain phenotype. In order to evaluate this possibility a technique was developed that combined autoradiography and immunocytochemistry. Results indicated that angiotensin II binding sites were not localized preferentially to either norepinephrine or epinephrine cells. Binding of angiotensin II was associated with the release of intracellular catecholamine stores. Cells were pre-loaded with /sup 3/H-norepinephrine and secretion was monitored by following radioactivity released into the supernatant. Alternatively, release of endogenous catecholamines was determined by fluorometric assay.

• Research Organization: California Univ., San Diego (USA)
• OSTI ID: 6154723
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ADRENAL HORMONES
AMINES
ANGIOTENSIN
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CATTLE
CHEMICAL COMPOSITION
DOMESTIC ANIMALS
DRUGS
ENDOCRINE GLANDS
GLANDS
GLOBULINS
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
LIGANDS
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHENOTYPE
POLYPHENOLS
PROTEINS
REACTION KINETICS
RECEPTORS
RUMINANTS
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES