Toluene model for hydrocarbon risk assessment. Final report, 1 January-31 December 1989

Technical Report · Tue May 29 00:00:00 EDT 1990

OSTI ID:6154014

Morre, J

This project was for continuation of research to investigate the molecular mode of action of a membrane-active hydrocarbon, toluene, potentially present in the Air Force environment as a flight fuel component or from other sources and to serve as a model for other membrane-active molecules in the environment. Two important target sites were identified where rapid dose-dependent but reversible changes in the membrane organization occurred at low dose levels. One of these was at the plasma membrane where the ability of the membrane to form protuberances was severely compromised. The other concerned a failure to form protuberances by membranes involved in internal trafficking between the endoplasmic reticulum and the Golgi apparatus. This step was reproduced in a cell-free system making detailed studies possible. The toluene inhibited step was identified as dependent on ATP hydrolysis. The involved ATPase activity was characterized, solubilized and partially purified.

• Research Organization: Purdue Univ., Lafayette, IN (USA). Dept. of Medicinal Chemistry and Pharmacognosy
• OSTI ID: 6154014
• Report Number(s): AD-A-225358/1/XAB; CNN: AFOSR-89-0219
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP-ASE
ENZYME ACTIVITY
TOLUENE
TOXICITY
ATP
CELL MEMBRANES
DOSE-RESPONSE RELATIONSHIPS
MAN
PROGRESS REPORT
RISK ASSESSMENT
ACID ANHYDRASES
ALKYLATED AROMATICS
ANIMALS
AROMATICS
CELL CONSTITUENTS
DOCUMENT TYPES
ENZYMES
HYDROCARBONS
HYDROLASES
MAMMALS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PRIMATES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology