K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.15.5983· OSTI ID:6153223

Hattori, Yutaka; Odagiri, Hiroki; Nakatani, Hiroshi; Miyagawa, Kiyoshi; Naito, Kenichiro; Sakamoto, Hiromi; Katoh, Osamu; Yoshida, Teruhiko; Sugimura, Takashi; Terada, Masaaki ^[1]

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1. National Cancer Center Research Institute, Tokyo (Japan)

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam ({und K}ATO-III cell-derived {und s}tomach cancer {und am}plified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

• OSTI ID: 6153223
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RECEPTORS
RECOMBINANT DNA
DNA SEQUENCING
TUMOR CELLS
GENE AMPLIFICATION
AMINO ACID SEQUENCE
DNA HYBRIDIZATION
DNA POLYMERASES
GENES
HEPARIN
NEOPLASMS
PHOSPHORUS 32
STOMACH
TRANSCRIPTION
AMINES
ANIMAL CELLS
ANTICOAGULANTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DNA
DRUGS
ENZYMES
GASTROINTESTINAL TRACT
HEMATOLOGIC AGENTS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
MOLECULAR STRUCTURE
MUCOPOLYSACCHARIDES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550200* - Biochemistry