Amiloride-inhibited Na -H exchange in human kidney medulla microsomes

Journal Article · Fri Aug 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:6151931

LaBelle, E F

Membrane vesicles from the medulla of the human kidney are capable of amiloride-inhibited sodium-proton exchange. Sodium uptake into the vesicles in stimulated by low intravesicular pH (6.0) and inhibited by both low extravesicular pH (6.0) and amiloride (2 mM). Sodium uptake is a linear function of the vesicular protein concentration and can be reversed by raising the osmotic strength of the solution to shrink the vesicle or by adding the inophore nigericin. Acridine orange was accumulated by the vesicles in response to a pH gradient (pH 6.0, inside, 8.0 outside), and the accumulation of acridine orange could be reduced by increase extravesicular sodium. Thus sodium could stimulate proton movement just as protons could stimulate sodium movement across the vesicular membrane.

• Research Organization: Univ. of Texas Medical Branch, Galveston
• OSTI ID: 6151931
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACRIDINE ORANGE
ACRIDINES
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
AMIDES
AMINES
ANIMALS
AROMATICS
AZAARENES
AZINES
BARYONS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHLORIDES
CHLORINE COMPOUNDS
DYES
ELEMENTARY PARTICLES
ENZYME ACTIVITY
ENZYMES
FERMIONS
HADRONS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HOMEOSTASIS
HYDROGEN IONS
HYDROGEN IONS 1 PLUS
IONS
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANE TRANSPORT
MICROSOMES
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PH VALUE
PHYSIOLOGY
PRIMATES
PROTONS
PYRIDINES
RADIOISOTOPES
SODIUM 22
SODIUM CHLORIDES
SODIUM COMPOUNDS
SODIUM ISOTOPES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES