Polycarbonate filter technique for collection of sorted cells
Journal Article
·
· Cytometry (Baltimore); (United States)
We describe the application of polycarbonate filters to the collection of cells sorted by flow cytometry. Cells are sorted directly onto polycarbonate filters and transferred to microscope slides which are then processed to dissolve the filter and remove pore outlines. The technique results in cell preparations which retain cellular morphology and have highcell recovery of 82 to 100%.
- Research Organization:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6149262
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 3:1
- Country of Publication:
- United States
- Language:
- English
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