Investigations of the mechanism of vitamin E removal from blood and the effect of NO/sub 2/ on the pulmonary uptake of vitamin E
To examine the effect of NO/sub 2/ on vitamin E uptake by lung, we first investigated the mechanism of removal of vitamin E from blood. To accomplish this, a method for labeling serum with tritiated vitamin E was developed. Two other lipids, whose uptake is better understood, were also studied, and compared with vitamin E. Tritiated triacylglycerol was used to monitor lipoprotein lipase-mediated uptake, while tritiated cholesterol was used to monitor LDL receptor-mediated uptake. Blood curves derived from untreated, adult, male, Long-Evans rats indicated that all three lipids were removed biexponentially. The half-life for the Rapid component was 2.5-3.7 minutes for all lipids. The slower curve had half-lives of 42.3 minutes for triacylglycerol, 990.0 minutes for cholesterol, and 288.8 minutes for vitamin E. Biological screening of labeled serum demonstrated that the faster process was not simply the removal of lipoproteins damaged by the labeling process. Hepatectomy affected the rate of neither triacylglycerol nor cholesterol removal, but did slow vitamin E uptake, suggesting a distinct role for liver in vitamin E extraction.
- Research Organization:
- Rochester Univ., NY (USA)
- OSTI ID:
- 6147642
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
NITROGEN DIOXIDE
BIOLOGICAL EFFECTS
VITAMIN E
UPTAKE
BIOLOGICAL HALF-LIFE
BLOOD
CHOLESTEROL
GLYCEROL
LIPASE
LIVER
LUNGS
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLESTERASES
CHALCOGENIDES
DIGESTIVE SYSTEM
ENZYMES
ESTERASES
GLANDS
HYDROLASES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
STEROIDS
STEROLS
VERTEBRATES
VITAMINS
560300* - Chemicals Metabolism & Toxicology
550501 - Metabolism- Tracer Techniques