Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions

Journal Article · Fri Nov 30 23:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.23.9133· OSTI ID:6144540

Matsumoto, Akiyo; Itakura, Hiroshige; Kodama, Tatsuhiko ^[1]; Naito, Makoto; Takahashi, Kiyoshi ^[2]; Ikemoto, Shinji; Asaoka, Hitoshi; Hayakawa, Ikuho ^[3]; Kanamori, Hiroshi; Takaku, Fumimaro ^[4]; Aburatani, Hiroyuki ^[5]; Suzuki, Hiroshi; Kobari, Yukage; Miyai, Tatsuya ^[6]; Cohen, E H; Wydro, R ^[7]; Housman, D E ^[8]

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1. Univ. of Tokyo (Japan) National Inst. of Health and Nutrition, Tokyo (Japan)
2. Kumamoto Univ. (Japan)
3. National Inst. of Health and Nutrition, Tokyo (Japan)
4. Univ. of Tokyo (Japan)
5. Univ. of Tokyo (Japan) Massachusetts Inst. of Tech., Cambridge, MA (United States)
6. Chugai Pharmaceutical, Tokyo (Japan)
7. Genzyme Corp., Framingham, MA (United States)
8. Massachusetts Inst. of Tech., Cambridge (United States)

Two types of cDNAs for human macrophage scavenger receptors were cloned from a cDNA library derived from the phorbol ester-treated human monocytic cell line THP-1. The type I and type II human scavenger receptors encoded by these cDNAs are homologous (73% and 71% amino acid identity) to their previously characterized bovine counterparts and consist of six domains: cytoplasmic (I), membrane-spanning (II), spacer (III), {alpha}-helical coiled-coil (IV), collagen-like (V), and a type-specific C-terminal (VI). The receptor gene is located on human chromosome 8. The human receptors expressed in CHO-K1 cells mediated endocytosis of modified low density lipoproteins. Two mRNAs, 4.0 and 3.2 kilobases, have been detected in human liver, placenta, and brain. Immunohistochemical studies using an anti-peptide antibody which recognizes human scavenger receptors indicated the presence of the scavenger receptors in the macrophages of lipid-rich atherosclerotic lesions, suggesting the involvement of scavenger receptors in atherogenesis.

• OSTI ID: 6144540
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:23; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMAL CELLS
ARTERIOSCLEROSIS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BODY
BRAIN
CARCINOGENS
CARDIOVASCULAR DISEASES
CENTRAL NERVOUS SYSTEM
CHEMISTRY
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ESTERS
FETAL MEMBRANES
GLANDS
HETEROCHROMOSOMES
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIVER
MACROPHAGES
MEMBRANE PROTEINS
MEMBRANES
MESSENGER-RNA
MOLECULAR STRUCTURE
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PHAGOCYTES
PHORBOL ESTERS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLACENTA
PROTEINS
RADIOISOTOPES
RECEPTORS
RECOMBINANT DNA
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VASCULAR DISEASES