Effect of agitation on ligninase activity and ligninase production by Phanerochaete chrysosporium

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6143350

Venkatadri, R; Irvine, R L ^[1]

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1. Univ. of Notre Dame, IN (USA)

The white rot fungus Phanerochaete chrysosporium produces extracellular ligninases as part of its idiophasic ligninolytic system. Agitation has been widely reported to suppress both ligninase production and lignin degradation. Results show that mechanical inactivation of ligninase is possibly the reason why ligninase accumulation is low or absent in agitated shake-flask cultures. Agitation seems to affect the catalytic activity of ligninase and has no apparent effect on either the rate of ligninase production or the physiology of P. chrysosporium. The detergents Tween 20, Tween 40, Tween 60, Tween 80, and 3-((3-cholamidopropyl)dimethylammonio)-1-propanesuflonate (CHAPS) are able to protect both purified ligninase and extant ligninase in culture fluids (free of biomass) against mechanical inactivation due to agitation. Addition of Tween 80 at the end of primary growth to agitated shake flasks containing either pelleted or immobilized mycelial cultures results in production and maintenance of high levels of ligninase activity over several days under conditions of high agitation. Possible mechanisms by which the detergents could protect ligninase are discussed.

• OSTI ID: 6143350
• Journal Information: Applied and Environmental Microbiology; (USA), Journal Name: Applied and Environmental Microbiology; (USA) Vol. 56:9; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ADDITIVES
BIODEGRADATION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DETERGENTS
EMULSIFIERS
ENZYME ACTIVITY
ENZYMES
FUNGI
INACTIVATION
LIGNIN
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SURFACTANTS
WETTING AGENTS