Involvement of protein kinase C in the modulation of morphine-induced analgesia and the inhibitory effects of exposure to 60-hz magnetic fields in the land snail, Cepaea nemoralis
- Univ. of Western Ontario, London (Canada)
One of the more consistent and dramatic effects of exposure to magnetic fields is the attenuation of morphine-induced analgesia. Results of previous studies have implicated alterations in calcium channel functioning and Ca{sup ++} flux in the mediation of these effects. It is generally accepted that Ca{sup ++}-activated-phospholipid-dependent protein kinase (Protein kinase C; PKC) plays an important role in relaying trans-membrane signaling in diverse Ca{sup ++} dependent cellular processes. In experiment 1 we observed that morphine-induced analgesia in the land snail, Cepaea nemoralis, as measured by the latency of an avoidance behavior to a warmed surface, was reduced by the PKC activator, SC-9, and was enhanced by the PKC inhibitors, H-7 and H-9. In contrast, HA-10004, a potent inhibitor of other protein kinases, but only a very weak inhibitor of PKC, had no effect on morphine-induced analgesia. In experiment 2 exposure of snails for 30 minutes to a 1.0 gauss (rms) 60-Hz magnetic field reduced morphine-induced analgesia. This inhibitory effect of the magnetic field was reduced by the PKC inhibitors, H-7 and H-9, and was augmented by the PKC activator SC-9. These results suggest that: (i) PKC is involved in the modulation of morphine-induced analgesia and, (ii) the inhibitory effects of magnetic fields involve PKC.
- OSTI ID:
- 6140616
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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MAGNETIC FIELDS
BIOLOGICAL EFFECTS
PHOSPHOTRANSFERASES
BIOCHEMICAL REACTION KINETICS
BEHAVIOR
CALCIUM COMPOUNDS
ENZYME INHIBITORS
ION CHANNELING
MORPHINE
SNAILS
ALKALINE EARTH METAL COMPOUNDS
ALKALOIDS
ANALGESICS
ANIMALS
AQUATIC ORGANISMS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHANNELING
DRUGS
ENZYMES
INVERTEBRATES
KINETICS
MOLLUSCS
NARCOTICS
OPIUM
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
REACTION KINETICS
TRANSFERASES
560400* - Other Environmental Pollutant Effects