Enzymatic transformation of hydrocarbons by methanotrophic organisms
Soluble methane monooxygenase from a facultative methane-utilizing organism, Methylobacterium sp. CRL-26 or R6, catalyzed the NAD(P)H-dependent epoxidation/hydroxylation of a variety of hydrocarbons, including terminal alkenes, internal alkenes, substituted alkenes, branch-chain alkenes, alkanes (C1-C8), substituted alkanes, branch-chain alkanes, carbon monoxide, ether, cyclic and aromatic compounds. The NAD -linked dehydrogenases such as formate dehydrogenase or secondary alcohol dehydrogenase in the presence of formate or secondary alcohol, respectively, regenerated NAD/NADH required for the methane monooxygenase in a coupled enzymes reactions. Oxidation of secondary alcohols to the corresponding methylketones in methanotrophs is catalyzed by an NAD -dependent, zinc-containing, secondary alcohol hydrogenase. Primary alcohols were oxidized to the corresponding aldehydes by a phenazine methosulfate-dependent, pyrollo quinoline quinone (methoxatin or PQQ) containing, methanol dehydrogenase. Oxidation of aldehydes (C1 to C10) to the corresponding carboxylic acids is catalyzed by a heme-containing aldehyde dehydrogenase. Methanotrophs have been considered potentially useful for single cell protein (SCP), amino acids, and biopolymer production at the expense of growth on cheap and readily available C1 compounds. 80 references, 1 figure, 6 tables.
- Research Organization:
- Exxon Research and Engineering Co., Linden, NJ
- OSTI ID:
- 6139418
- Report Number(s):
- CONF-8208164-
- Journal Information:
- Dev. Ind. Microbiol.; (United States), Journal Name: Dev. Ind. Microbiol.; (United States) Vol. 24; ISSN DIMCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
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ALCOHOLS
ALDEHYDES
ALKANES
ALKENES
AMINO ACIDS
AROMATICS
BACTERIA
BIOCONVERSION
BIOSYNTHESIS
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CHALCOGENIDES
COENZYMES
DATA
ENZYME ACTIVITY
ENZYMES
ETHERS
EXPERIMENTAL DATA
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
METHANOL
METHANOTROPHIC BACTERIA
MICROORGANISMS
NAD
NADP
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
POLYMERS
SINGLE CELL PROTEIN
SYNTHESIS